Although the personalized microrobots we conceptualized can significantly reduce immunogenicity-related issues, regardless of the high proportions of patient materials we use, these microrobots still include additional synthetic components, such as magnetic nanoparticles and photoinitiators, which may still be recognized by the immune system. Our recent research has applied a new concept in the microrobotics field a personalized medicine approach through patient-blood derivable biomaterials in the fabrication of microrobots to minimize safety risks and unintended immune responses. However, such synthetic and biohybrid microrobots cannot be long-term deployed in the body since they are typically deleterious and promptly cleared by the body's immune system, where the failure may lead to the formation of granulomas and inflammation. So far, various mobile microrobotic platforms have been developed using versatile synthetic materials or self-propelled motile microorganisms (e.g., bacteria and microalgae) that have been further modified with functional units for a targeted medical application. Untethered mobile microrobots have significant potential to access hard-to-reach, deep, and risky human body regions for minimally invasive medical interventions. This study further demonstrates such immunobots from freshly isolated primary bone marrow-derived macrophages since patient-derivable macrophages may have a strong clinical potential for future cell therapies in cancer. The immunobots exhibit anticancer activity against urinary bladder cancer cells. The immunobots secrete signature M1 cytokines, IL-12 p40, TNF-α, and IL-6, and M1 cell markers, CD80 and iNOS, via toll-like receptor 4 (TLR4)-mediated stimulation with bacterial LPS. This study demonstrates the torque-based surface rolling locomotion of the immunobots along assigned trajectories inside blood plasma, over a layer of endothelial cells, and under physiologically relevant flow rates. Macrophages engulf the engineered magnetic decoy bacteria, composed of 0.5 µm diameter silica Janus particles with one side coated with anisotropic FePt magnetic nanofilm and the other side coated with bacterial lipopolysaccharide (LPS). Here, live immune cell-derived microrobots from macrophages, i.e., immunobots, which can be remotely steered with externally applied magnetic fields and directed toward anti-tumorigenic (M1) phenotypes, are presented. More importantly, live cells can enable therapeutically relevant functions with significantly higher efficiency than synthetic systems. Please contact us with any questions regarding the facility, flow cytometry, or the development of new applications.Building medical microrobots from the body's own cells may circumvent the biocompatibility concern and hence presents more potential in clinical applications to improve the possibility of escaping from the host defense mechanism. After hours or weekend access is permitted for trained UConn Health employees. The facility is staffed Monday through Friday from 9 a.m.
#FLOWJO ACQUIRED BY BD PRO#
These include: FlowJo (Treestar Inc.), BD FACS DIVA (BD Bioscience), Cell Quest Pro (BD Bioscience), ModFit LT (Verity), and FCAP Array (BD Bioscience). We also provide several computer workstations with flow cytometry and cytometric bead array analysis software. Additionally, the 15 parameter FACS ARIA II is installed in a Baker Bioprotect III safety cabinet, which facilitates analysis and sorting of potentially biohazardous samples. Our FACS ARIA II cell sorters are both equipped with five lasers one can analyze 15 parameters, and the other is capable of 18 parameter analysis. One of our LSR II instruments is equipped with four lasers and is capable of 13 parameter analysis. Two of our LSR II instruments are equipped with five lasers and are capable of 15 or 18 parameter analysis. We house two four-color BD FACS Calibur instruments, three BD LSR II instruments, a MACSQuant10, and two BD FACS ARIA II high speed cell sorters.
#FLOWJO ACQUIRED BY BD PORTABLE#
Data should be transferred to this location or to the user's own portable drive after completion of an experiment.
The server link is available on the desktop of each computer. Instrument computers can also be used for data analysis, but data acquisition takes precedence over data analysis.Ī centralized flow cytometry server is available for downloading data off of the instruments. As a centralized resource, the flow cytometry facility provides researchers and clinical investigators at UConn Health and surrounding institutions with the ability to perform both multi-parameter analysis and sorting of cell populations into phenotypic, biochemical, and molecular subsets, based on a variety of characteristics including size and shape and fluorescence derived from antibody-tagging or expression of fluorescent molecules.Ĭomputer workstations are available for data analysis.
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